Ernesto Valverde’s men take on their La Liga rivals in the Copa del Rey quarter-finals first leg.
Boateng joined Barcelona on loan earlier this week and has been immediately preferred to the 25-year-old.
Suarez has been heavily linked with a transfer to the Gunners this month and Barcelona’s announcement will only add to the speculation surrounding the midfielder’s future.
Barcelona broke the news on Twitter, saying: “Following confirmation of the transfer of Boateng, there are 18 players in the squad list to face Sevilla FC. Denis Suárez is the player who misses out.”